Virulence of different Agrobacterium strains on hairy root formation of Hyoscyamus muticus

Leena Vanhala; Raimo Hiltunen; Kirsi Marja Oksman-Caldentey

doi:10.1007/BF00233640

Virulence of different Agrobacterium strains on hairy root formation of Hyoscyamus muticus

Leena Vanhala^*, Raimo Hiltunen, Kirsi Marja Oksman-Caldentey

^*Corresponding author for this work

Not published at VTT

University of Helsinki

Research output: Contribution to journal › Article › Scientific › peer-review

47 Citations (Scopus)

Abstract

Hyoscyamus muticus accession was evaluated for its response to inoculation with Agrobacterium rhizogenes strains LBA9402, A4, 15834, and Agrobacterium tumefaciens strain C58 CI pRT GUS104. The Agrobacterium strain used for the transformation has a significant influence on the phenotype of the clone as well as on the growth rate and hyoscyamine production of these root culture clones. The most virulent strains were C58 CI pRT GUS104 and LBA9402. More roots were obtained on LSO medium than on B50 medium. Acetosyringone addition and the time from wounding affected root formation. The alkaloid content was highest in clones C58 and A4 (≈90mg·l^-1). There are great differences between individual hairy root clones, and hence they are not as uniform as has often been speculated. The Agrobacterium strain used for the transformation has a great influence in this respect.

Original language	English
Pages (from-to)	236-240
Number of pages	5
Journal	Plant Cell Reports
Volume	14
Issue number	4
DOIs	https://doi.org/10.1007/BF00233640
Publication status	Published - 1 Jan 1995
MoE publication type	A1 Journal article-refereed

Keywords

Agrobacterium rhizogenes
hyoscyamine
Hyoscyamus muticus
transformed roots
virulence

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10.1007/BF00233640

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title = "Virulence of different Agrobacterium strains on hairy root formation of Hyoscyamus muticus",

abstract = "Hyoscyamus muticus accession was evaluated for its response to inoculation with Agrobacterium rhizogenes strains LBA9402, A4, 15834, and Agrobacterium tumefaciens strain C58 CI pRT GUS104. The Agrobacterium strain used for the transformation has a significant influence on the phenotype of the clone as well as on the growth rate and hyoscyamine production of these root culture clones. The most virulent strains were C58 CI pRT GUS104 and LBA9402. More roots were obtained on LSO medium than on B50 medium. Acetosyringone addition and the time from wounding affected root formation. The alkaloid content was highest in clones C58 and A4 (≈90mg·l-1). There are great differences between individual hairy root clones, and hence they are not as uniform as has often been speculated. The Agrobacterium strain used for the transformation has a great influence in this respect.",

keywords = "Agrobacterium rhizogenes, hyoscyamine, Hyoscyamus muticus, transformed roots, virulence",

author = "Leena Vanhala and Raimo Hiltunen and Oksman-Caldentey, {Kirsi Marja}",

year = "1995",

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doi = "10.1007/BF00233640",

language = "English",

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TY - JOUR

T1 - Virulence of different Agrobacterium strains on hairy root formation of Hyoscyamus muticus

AU - Vanhala, Leena

AU - Hiltunen, Raimo

AU - Oksman-Caldentey, Kirsi Marja

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Hyoscyamus muticus accession was evaluated for its response to inoculation with Agrobacterium rhizogenes strains LBA9402, A4, 15834, and Agrobacterium tumefaciens strain C58 CI pRT GUS104. The Agrobacterium strain used for the transformation has a significant influence on the phenotype of the clone as well as on the growth rate and hyoscyamine production of these root culture clones. The most virulent strains were C58 CI pRT GUS104 and LBA9402. More roots were obtained on LSO medium than on B50 medium. Acetosyringone addition and the time from wounding affected root formation. The alkaloid content was highest in clones C58 and A4 (≈90mg·l-1). There are great differences between individual hairy root clones, and hence they are not as uniform as has often been speculated. The Agrobacterium strain used for the transformation has a great influence in this respect.

AB - Hyoscyamus muticus accession was evaluated for its response to inoculation with Agrobacterium rhizogenes strains LBA9402, A4, 15834, and Agrobacterium tumefaciens strain C58 CI pRT GUS104. The Agrobacterium strain used for the transformation has a significant influence on the phenotype of the clone as well as on the growth rate and hyoscyamine production of these root culture clones. The most virulent strains were C58 CI pRT GUS104 and LBA9402. More roots were obtained on LSO medium than on B50 medium. Acetosyringone addition and the time from wounding affected root formation. The alkaloid content was highest in clones C58 and A4 (≈90mg·l-1). There are great differences between individual hairy root clones, and hence they are not as uniform as has often been speculated. The Agrobacterium strain used for the transformation has a great influence in this respect.

KW - Agrobacterium rhizogenes

KW - hyoscyamine

KW - Hyoscyamus muticus

KW - transformed roots

KW - virulence

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U2 - 10.1007/BF00233640

DO - 10.1007/BF00233640

M3 - Article

AN - SCOPUS:0029136841

SN - 0721-7714

VL - 14

SP - 236

EP - 240

JO - Plant Cell Reports

JF - Plant Cell Reports

IS - 4

ER -

Virulence of different Agrobacterium strains on hairy root formation of Hyoscyamus muticus

Abstract

Keywords

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