Virulence of different Agrobacterium strains on hairy root formation of Hyoscyamus muticus

Leena Vanhala, Raimo Hiltunen, Kirsi Marja Oksman-Caldentey

Research output: Contribution to journalArticleScientificpeer-review

36 Citations (Scopus)

Abstract

Hyoscyamus muticus accession was evaluated for its response to inoculation with Agrobacterium rhizogenes strains LBA9402, A4, 15834, and Agrobacterium tumefaciens strain C58 CI pRT GUS104. The Agrobacterium strain used for the transformation has a significant influence on the phenotype of the clone as well as on the growth rate and hyoscyamine production of these root culture clones. The most virulent strains were C58 CI pRT GUS104 and LBA9402. More roots were obtained on LSO medium than on B50 medium. Acetosyringone addition and the time from wounding affected root formation. The alkaloid content was highest in clones C58 and A4 (≈90mg·l-1). There are great differences between individual hairy root clones, and hence they are not as uniform as has often been speculated. The Agrobacterium strain used for the transformation has a great influence in this respect.

Original languageEnglish
Pages (from-to)236-240
Number of pages5
JournalPlant Cell Reports
Volume14
Issue number4
DOIs
Publication statusPublished - 1 Jan 1995
MoE publication typeA1 Journal article-refereed

Fingerprint

Hyoscyamus muticus
Agrobacterium
virulence
clones
acetosyringone
Rhizobium rhizogenes
atropine
Agrobacterium radiobacter
alkaloids
phenotype

Keywords

  • Agrobacterium rhizogenes
  • hyoscyamine
  • Hyoscyamus muticus
  • transformed roots
  • virulence

Cite this

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title = "Virulence of different Agrobacterium strains on hairy root formation of Hyoscyamus muticus",
abstract = "Hyoscyamus muticus accession was evaluated for its response to inoculation with Agrobacterium rhizogenes strains LBA9402, A4, 15834, and Agrobacterium tumefaciens strain C58 CI pRT GUS104. The Agrobacterium strain used for the transformation has a significant influence on the phenotype of the clone as well as on the growth rate and hyoscyamine production of these root culture clones. The most virulent strains were C58 CI pRT GUS104 and LBA9402. More roots were obtained on LSO medium than on B50 medium. Acetosyringone addition and the time from wounding affected root formation. The alkaloid content was highest in clones C58 and A4 (≈90mg·l-1). There are great differences between individual hairy root clones, and hence they are not as uniform as has often been speculated. The Agrobacterium strain used for the transformation has a great influence in this respect.",
keywords = "Agrobacterium rhizogenes, hyoscyamine, Hyoscyamus muticus, transformed roots, virulence",
author = "Leena Vanhala and Raimo Hiltunen and Oksman-Caldentey, {Kirsi Marja}",
year = "1995",
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pages = "236--240",
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Virulence of different Agrobacterium strains on hairy root formation of Hyoscyamus muticus. / Vanhala, Leena; Hiltunen, Raimo; Oksman-Caldentey, Kirsi Marja.

In: Plant Cell Reports, Vol. 14, No. 4, 01.01.1995, p. 236-240.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Vanhala, Leena

AU - Hiltunen, Raimo

AU - Oksman-Caldentey, Kirsi Marja

PY - 1995/1/1

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N2 - Hyoscyamus muticus accession was evaluated for its response to inoculation with Agrobacterium rhizogenes strains LBA9402, A4, 15834, and Agrobacterium tumefaciens strain C58 CI pRT GUS104. The Agrobacterium strain used for the transformation has a significant influence on the phenotype of the clone as well as on the growth rate and hyoscyamine production of these root culture clones. The most virulent strains were C58 CI pRT GUS104 and LBA9402. More roots were obtained on LSO medium than on B50 medium. Acetosyringone addition and the time from wounding affected root formation. The alkaloid content was highest in clones C58 and A4 (≈90mg·l-1). There are great differences between individual hairy root clones, and hence they are not as uniform as has often been speculated. The Agrobacterium strain used for the transformation has a great influence in this respect.

AB - Hyoscyamus muticus accession was evaluated for its response to inoculation with Agrobacterium rhizogenes strains LBA9402, A4, 15834, and Agrobacterium tumefaciens strain C58 CI pRT GUS104. The Agrobacterium strain used for the transformation has a significant influence on the phenotype of the clone as well as on the growth rate and hyoscyamine production of these root culture clones. The most virulent strains were C58 CI pRT GUS104 and LBA9402. More roots were obtained on LSO medium than on B50 medium. Acetosyringone addition and the time from wounding affected root formation. The alkaloid content was highest in clones C58 and A4 (≈90mg·l-1). There are great differences between individual hairy root clones, and hence they are not as uniform as has often been speculated. The Agrobacterium strain used for the transformation has a great influence in this respect.

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