Visual observation of hydrolyzed potato starch granules by alfa-amylase with confocal laser scanning microscopy

Soottitantawat Apinan; Ikuta Yujiro; Yoshii Hidefumi; Furuta Takeshi; Päivi Myllärinen; Pirkko Forssell; Kaisa Poutanen

doi:10.1002/star.200700630

Visual observation of hydrolyzed potato starch granules by alfa-amylase with confocal laser scanning microscopy

Soottitantawat Apinan, Ikuta Yujiro, Yoshii Hidefumi (Corresponding Author), Furuta Takeshi, Päivi Myllärinen, Pirkko Forssell, Kaisa Poutanen

Research output: Contribution to journal › Article › Scientific › peer-review

33 Citations (Scopus)

Abstract

Porous starch was produced by digestion of freeze-dried potato starch with alfa-amylase from Bacillus sp. The surface structure of the granules became perforated and in the interior of the granules a capsule-like cavity was formed, i.e. the hydrolyzed starch can be used as an encapsulant. The structure change of the granules was observed with confocal laser scanning microscopy and scanning electron microscopy. The degree of starch hydrolysis could be correlated with the Avrami equation. The activation energy of starch hydrolysis by alfa-amylase was 83 kJ/mol.

Original language	English
Pages (from-to)	543-548
Journal	Stärke
Volume	59
Issue number	11
DOIs	https://doi.org/10.1002/star.200700630
Publication status	Published - 2007
MoE publication type	A1 Journal article-refereed

Keywords

alfa-amylase
starch granule
cavity
CLSM
SEM

Access to Document

10.1002/star.200700630

Cite this

@article{da2b19f56b4d4d3bb6767e08ac716338,

title = "Visual observation of hydrolyzed potato starch granules by alfa-amylase with confocal laser scanning microscopy",

abstract = "Porous starch was produced by digestion of freeze-dried potato starch with alfa-amylase from Bacillus sp. The surface structure of the granules became perforated and in the interior of the granules a capsule-like cavity was formed, i.e. the hydrolyzed starch can be used as an encapsulant. The structure change of the granules was observed with confocal laser scanning microscopy and scanning electron microscopy. The degree of starch hydrolysis could be correlated with the Avrami equation. The activation energy of starch hydrolysis by alfa-amylase was 83 kJ/mol.",

keywords = "alfa-amylase, starch granule, cavity, CLSM, SEM",

author = "Soottitantawat Apinan and Ikuta Yujiro and Yoshii Hidefumi and Furuta Takeshi and P{\"a}ivi Myll{\"a}rinen and Pirkko Forssell and Kaisa Poutanen",

year = "2007",

doi = "10.1002/star.200700630",

language = "English",

volume = "59",

pages = "543--548",

journal = "St{\"a}rke",

issn = "0038-9056",

publisher = "Wiley-VCH Verlag",

number = "11",

}

TY - JOUR

T1 - Visual observation of hydrolyzed potato starch granules by alfa-amylase with confocal laser scanning microscopy

AU - Apinan, Soottitantawat

AU - Yujiro, Ikuta

AU - Hidefumi, Yoshii

AU - Takeshi, Furuta

AU - Myllärinen, Päivi

AU - Forssell, Pirkko

AU - Poutanen, Kaisa

PY - 2007

Y1 - 2007

N2 - Porous starch was produced by digestion of freeze-dried potato starch with alfa-amylase from Bacillus sp. The surface structure of the granules became perforated and in the interior of the granules a capsule-like cavity was formed, i.e. the hydrolyzed starch can be used as an encapsulant. The structure change of the granules was observed with confocal laser scanning microscopy and scanning electron microscopy. The degree of starch hydrolysis could be correlated with the Avrami equation. The activation energy of starch hydrolysis by alfa-amylase was 83 kJ/mol.

AB - Porous starch was produced by digestion of freeze-dried potato starch with alfa-amylase from Bacillus sp. The surface structure of the granules became perforated and in the interior of the granules a capsule-like cavity was formed, i.e. the hydrolyzed starch can be used as an encapsulant. The structure change of the granules was observed with confocal laser scanning microscopy and scanning electron microscopy. The degree of starch hydrolysis could be correlated with the Avrami equation. The activation energy of starch hydrolysis by alfa-amylase was 83 kJ/mol.

KW - alfa-amylase

KW - starch granule

KW - cavity

KW - CLSM

KW - SEM

U2 - 10.1002/star.200700630

DO - 10.1002/star.200700630

M3 - Article

SN - 0038-9056

VL - 59

SP - 543

EP - 548

JO - Stärke

JF - Stärke

IS - 11

ER -

Visual observation of hydrolyzed potato starch granules by alfa-amylase with confocal laser scanning microscopy

Abstract

Keywords

Access to Document

Fingerprint

Cite this