Visual observation of hydrolyzed potato starch granules by alfa-amylase with confocal laser scanning microscopy

Soottitantawat Apinan, Ikuta Yujiro, Yoshii Hidefumi (Corresponding Author), Furuta Takeshi, Päivi Myllärinen, Pirkko Forssell, Kaisa Poutanen

Research output: Contribution to journalArticleScientificpeer-review

22 Citations (Scopus)

Abstract

Porous starch was produced by digestion of freeze-dried potato starch with alfa-amylase from Bacillus sp. The surface structure of the granules became perforated and in the interior of the granules a capsule-like cavity was formed, i.e. the hydrolyzed starch can be used as an encapsulant. The structure change of the granules was observed with confocal laser scanning microscopy and scanning electron microscopy. The degree of starch hydrolysis could be correlated with the Avrami equation. The activation energy of starch hydrolysis by alfa-amylase was 83 kJ/mol.
Original languageEnglish
Pages (from-to)543-548
JournalStärke
Volume59
Issue number11
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

Fingerprint

alpha-Amylases
potato starch
confocal laser scanning microscopy
Solanum tuberosum
starch granules
alpha-amylase
Confocal Microscopy
Starch
Microscopic examination
Observation
starch
Scanning
granules
Lasers
hydrolysis
Hydrolysis
activation energy
Bacilli
scanning electron microscopy
Surface structure

Keywords

  • alfa-amylase
  • starch granule
  • cavity
  • CLSM
  • SEM

Cite this

Apinan, Soottitantawat ; Yujiro, Ikuta ; Hidefumi, Yoshii ; Takeshi, Furuta ; Myllärinen, Päivi ; Forssell, Pirkko ; Poutanen, Kaisa. / Visual observation of hydrolyzed potato starch granules by alfa-amylase with confocal laser scanning microscopy. In: Stärke. 2007 ; Vol. 59, No. 11. pp. 543-548.
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abstract = "Porous starch was produced by digestion of freeze-dried potato starch with alfa-amylase from Bacillus sp. The surface structure of the granules became perforated and in the interior of the granules a capsule-like cavity was formed, i.e. the hydrolyzed starch can be used as an encapsulant. The structure change of the granules was observed with confocal laser scanning microscopy and scanning electron microscopy. The degree of starch hydrolysis could be correlated with the Avrami equation. The activation energy of starch hydrolysis by alfa-amylase was 83 kJ/mol.",
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Apinan, S, Yujiro, I, Hidefumi, Y, Takeshi, F, Myllärinen, P, Forssell, P & Poutanen, K 2007, 'Visual observation of hydrolyzed potato starch granules by alfa-amylase with confocal laser scanning microscopy', Stärke, vol. 59, no. 11, pp. 543-548. https://doi.org/10.1002/star.200700630

Visual observation of hydrolyzed potato starch granules by alfa-amylase with confocal laser scanning microscopy. / Apinan, Soottitantawat; Yujiro, Ikuta; Hidefumi, Yoshii (Corresponding Author); Takeshi, Furuta; Myllärinen, Päivi; Forssell, Pirkko; Poutanen, Kaisa.

In: Stärke, Vol. 59, No. 11, 2007, p. 543-548.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Visual observation of hydrolyzed potato starch granules by alfa-amylase with confocal laser scanning microscopy

AU - Apinan, Soottitantawat

AU - Yujiro, Ikuta

AU - Hidefumi, Yoshii

AU - Takeshi, Furuta

AU - Myllärinen, Päivi

AU - Forssell, Pirkko

AU - Poutanen, Kaisa

PY - 2007

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N2 - Porous starch was produced by digestion of freeze-dried potato starch with alfa-amylase from Bacillus sp. The surface structure of the granules became perforated and in the interior of the granules a capsule-like cavity was formed, i.e. the hydrolyzed starch can be used as an encapsulant. The structure change of the granules was observed with confocal laser scanning microscopy and scanning electron microscopy. The degree of starch hydrolysis could be correlated with the Avrami equation. The activation energy of starch hydrolysis by alfa-amylase was 83 kJ/mol.

AB - Porous starch was produced by digestion of freeze-dried potato starch with alfa-amylase from Bacillus sp. The surface structure of the granules became perforated and in the interior of the granules a capsule-like cavity was formed, i.e. the hydrolyzed starch can be used as an encapsulant. The structure change of the granules was observed with confocal laser scanning microscopy and scanning electron microscopy. The degree of starch hydrolysis could be correlated with the Avrami equation. The activation energy of starch hydrolysis by alfa-amylase was 83 kJ/mol.

KW - alfa-amylase

KW - starch granule

KW - cavity

KW - CLSM

KW - SEM

U2 - 10.1002/star.200700630

DO - 10.1002/star.200700630

M3 - Article

VL - 59

SP - 543

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JO - Starch

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