Visualization of cellobiohydrolase i from trichoderma reesei moving on crystalline cellulose using high-speed atomic force microscopy

Kiyohiko Igarashi; Takayuki Uchihashi; Anu Koivula; Masahisa Wada; Satoshi Kimura; Merja Penttilä; Toshio Ando; Masahiro Samejima

doi:10.1016/B978-0-12-415931-0.00009-4

Visualization of cellobiohydrolase i from trichoderma reesei moving on crystalline cellulose using high-speed atomic force microscopy

Kiyohiko Igarashi, Takayuki Uchihashi, Anu Koivula, Masahisa Wada, Satoshi Kimura, Merja Penttilä, Toshio Ando, Masahiro Samejima

Research output: Contribution to journal › Article › Scientific › peer-review

15 Citations (Scopus)

Abstract

Cellulases hydrolyze β-1,4-glucosidic linkages of insoluble cellulose at the solid/liquid interface, generating soluble cellooligosaccharides. We describe here our method for real-time observation of the behavior of cellulase molecules on the substrate, using high-speed atomic force microscopy (HS-AFM). When glycoside hydrolase family 7 cellobiohydrolase from Trichoderma reesei (TrCel7A) was incubated with crystalline cellulose, many enzyme molecules were observed to move unidirectionally on the surface of the substrate by HS-AFM. The velocity of the moving molecules of TrCel7A on cellulose I crystals was estimated by means of image analysis.

Original language	English
Pages (from-to)	169-182
Journal	Methods in Enzymology
Volume	510
DOIs	https://doi.org/10.1016/B978-0-12-415931-0.00009-4
Publication status	Published - 22 May 2012
MoE publication type	A1 Journal article-refereed

Keywords

Biomass utilization
Cellulase
Cellulose
High-speed atomic force microscopy
Single-molecule observations
Trichoderma reesei

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Igarashi, K., Uchihashi, T., Koivula, A., Wada, M., Kimura, S., Penttilä, M., Ando, T., & Samejima, M. (2012). Visualization of cellobiohydrolase i from trichoderma reesei moving on crystalline cellulose using high-speed atomic force microscopy. Methods in Enzymology, 510, 169-182. https://doi.org/10.1016/B978-0-12-415931-0.00009-4

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abstract = "Cellulases hydrolyze β-1,4-glucosidic linkages of insoluble cellulose at the solid/liquid interface, generating soluble cellooligosaccharides. We describe here our method for real-time observation of the behavior of cellulase molecules on the substrate, using high-speed atomic force microscopy (HS-AFM). When glycoside hydrolase family 7 cellobiohydrolase from Trichoderma reesei (TrCel7A) was incubated with crystalline cellulose, many enzyme molecules were observed to move unidirectionally on the surface of the substrate by HS-AFM. The velocity of the moving molecules of TrCel7A on cellulose I crystals was estimated by means of image analysis.",

keywords = "Biomass utilization, Cellulase, Cellulose, High-speed atomic force microscopy, Single-molecule observations, Trichoderma reesei",

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Visualization of cellobiohydrolase i from trichoderma reesei moving on crystalline cellulose using high-speed atomic force microscopy. / Igarashi, Kiyohiko; Uchihashi, Takayuki; Koivula, Anu; Wada, Masahisa; Kimura, Satoshi; Penttilä, Merja; Ando, Toshio; Samejima, Masahiro.

In: Methods in Enzymology, Vol. 510, 22.05.2012, p. 169-182.

Research output: Contribution to journal › Article › Scientific › peer-review

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AU - Igarashi, Kiyohiko

AU - Uchihashi, Takayuki

AU - Koivula, Anu

AU - Wada, Masahisa

AU - Kimura, Satoshi

AU - Penttilä, Merja

AU - Ando, Toshio

AU - Samejima, Masahiro

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AB - Cellulases hydrolyze β-1,4-glucosidic linkages of insoluble cellulose at the solid/liquid interface, generating soluble cellooligosaccharides. We describe here our method for real-time observation of the behavior of cellulase molecules on the substrate, using high-speed atomic force microscopy (HS-AFM). When glycoside hydrolase family 7 cellobiohydrolase from Trichoderma reesei (TrCel7A) was incubated with crystalline cellulose, many enzyme molecules were observed to move unidirectionally on the surface of the substrate by HS-AFM. The velocity of the moving molecules of TrCel7A on cellulose I crystals was estimated by means of image analysis.

KW - Biomass utilization

KW - Cellulase

KW - Cellulose

KW - High-speed atomic force microscopy

KW - Single-molecule observations

KW - Trichoderma reesei

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