Water soluble and insoluble fractions or rye bran have distinct effects on adenoma growth in min mice

S. Oikarinen, E. Päivärinta, A. Sivula, Sirpa Karppinen, Kaisa Poutanen, M. Mutanen

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Rye is a promising candidate for anticarcinogenic components in human diet.Two recent studies using different animal models both suggest that rye bran may contain substances which prevent intestinal tumour formation in animals (Davies et al.Carcinogenesis 1999;20:927-931; Mutanen et al. Carcinogenesis 2000;21:1167-1173).In this study, we wanted to see if there exist differences between whole bran and water soluble (WS) and water insoluble (WIS) fractions of rye bran on intestinal adenoma formation in adenomatous polyposis coli (APC) knockout mice.These mice spontaneously develop multiple intestinal neoplasia (Min).Male heterozygous Min mice (n = 11-12/group) were fed one of the following high-fat AIN93G-based diets for five to six weeks: a casein based diet with 10% (w/w) rye bran, or 7.9% (w/w) WS or WIS fractions of rye bran.This way all three diets had the same amount of undigestible components (4.14%, w/w).In rye bran and in the WIS fraction dietary fiber and fructan contents were 34.3/7.1% and 51.1/1.2%, respectively. The WS fraction was prepared using xylanase treatment and it contained mainly fructan, pentosane-oligosacharides, and beta-glucan (21.2/26.7/4.4%).The fat (40% of energy) used in the diets was a mixture of butter, rapeseed oil, and sunflower seed oil.The intake of fatty acids corresponded to that in the Western type diet.The rye bran, WIS and WS diets produced similar number of adenomas in the small intestine (37±28; 38±34; 38±30, mean±SD) and in the colon+caecum (0.8±1.0; 1.0±1.1; 0.7±0.6).Opposite effect between WIS and WS diets were, however, seen on adenoma growth when comparing duodenum and the distal small intestine.In the duodenum the mean size of adenomas were 2.22±0.35 mm and 1.42±0.70 mm after the WIS and WS diets (p < 0.05, Mann-Whitney) while the corresponding values in the distal part of the small intestine were 0.99±0.21 mm and 1.20±0.17 mm (p < 0.05).The number of large (>1.5 mm) adenomas either in the duodenum or in the distal small intestine supported these results; in the duodenum WIS diet significantly increased the number of large adenomas (% of total) when compared with the whole rye bran (35±13% vs. 17±17%, p < 0.05), while it decreased the number of large adenomas in the distal small intestine (1.0±0.4% vs. 17±14%, p = 0.052).The results suggest that both WIS and WS fractions of rye bran probably contain substances which when separated from whole bran may, depending on the site of the intestine, have either favourable or unfavourable effects on the growth of adenomas in Min mice.
Original languageEnglish
Title of host publicationWhole Grain and Human Health: International Symposium
Subtitle of host publicationAbstracts
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages143-144
ISBN (Electronic)951-38-5719-0
ISBN (Print)951-38-5718-2
Publication statusPublished - 2001
EventWhole Grain and Human Health: International Symposium
- Porvoo, Finland
Duration: 13 Jun 200115 Jun 2001

Publication series

SeriesVTT Symposium
Number213
ISSN0357-9387

Conference

ConferenceWhole Grain and Human Health: International Symposium
CountryFinland
CityPorvoo
Period13/06/0115/06/01

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rye bran
adenoma
mice
water
diet
small intestine
duodenum
neoplasms
fructans
bran
carcinogenesis

Cite this

Oikarinen, S., Päivärinta, E., Sivula, A., Karppinen, S., Poutanen, K., & Mutanen, M. (2001). Water soluble and insoluble fractions or rye bran have distinct effects on adenoma growth in min mice. In Whole Grain and Human Health: International Symposium: Abstracts (pp. 143-144). Espoo: VTT Technical Research Centre of Finland. VTT Symposium, No. 213
Oikarinen, S. ; Päivärinta, E. ; Sivula, A. ; Karppinen, Sirpa ; Poutanen, Kaisa ; Mutanen, M. / Water soluble and insoluble fractions or rye bran have distinct effects on adenoma growth in min mice. Whole Grain and Human Health: International Symposium: Abstracts. Espoo : VTT Technical Research Centre of Finland, 2001. pp. 143-144 (VTT Symposium; No. 213).
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title = "Water soluble and insoluble fractions or rye bran have distinct effects on adenoma growth in min mice",
abstract = "Rye is a promising candidate for anticarcinogenic components in human diet.Two recent studies using different animal models both suggest that rye bran may contain substances which prevent intestinal tumour formation in animals (Davies et al.Carcinogenesis 1999;20:927-931; Mutanen et al. Carcinogenesis 2000;21:1167-1173).In this study, we wanted to see if there exist differences between whole bran and water soluble (WS) and water insoluble (WIS) fractions of rye bran on intestinal adenoma formation in adenomatous polyposis coli (APC) knockout mice.These mice spontaneously develop multiple intestinal neoplasia (Min).Male heterozygous Min mice (n = 11-12/group) were fed one of the following high-fat AIN93G-based diets for five to six weeks: a casein based diet with 10{\%} (w/w) rye bran, or 7.9{\%} (w/w) WS or WIS fractions of rye bran.This way all three diets had the same amount of undigestible components (4.14{\%}, w/w).In rye bran and in the WIS fraction dietary fiber and fructan contents were 34.3/7.1{\%} and 51.1/1.2{\%}, respectively. The WS fraction was prepared using xylanase treatment and it contained mainly fructan, pentosane-oligosacharides, and beta-glucan (21.2/26.7/4.4{\%}).The fat (40{\%} of energy) used in the diets was a mixture of butter, rapeseed oil, and sunflower seed oil.The intake of fatty acids corresponded to that in the Western type diet.The rye bran, WIS and WS diets produced similar number of adenomas in the small intestine (37±28; 38±34; 38±30, mean±SD) and in the colon+caecum (0.8±1.0; 1.0±1.1; 0.7±0.6).Opposite effect between WIS and WS diets were, however, seen on adenoma growth when comparing duodenum and the distal small intestine.In the duodenum the mean size of adenomas were 2.22±0.35 mm and 1.42±0.70 mm after the WIS and WS diets (p < 0.05, Mann-Whitney) while the corresponding values in the distal part of the small intestine were 0.99±0.21 mm and 1.20±0.17 mm (p < 0.05).The number of large (>1.5 mm) adenomas either in the duodenum or in the distal small intestine supported these results; in the duodenum WIS diet significantly increased the number of large adenomas ({\%} of total) when compared with the whole rye bran (35±13{\%} vs. 17±17{\%}, p < 0.05), while it decreased the number of large adenomas in the distal small intestine (1.0±0.4{\%} vs. 17±14{\%}, p = 0.052).The results suggest that both WIS and WS fractions of rye bran probably contain substances which when separated from whole bran may, depending on the site of the intestine, have either favourable or unfavourable effects on the growth of adenomas in Min mice.",
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Oikarinen, S, Päivärinta, E, Sivula, A, Karppinen, S, Poutanen, K & Mutanen, M 2001, Water soluble and insoluble fractions or rye bran have distinct effects on adenoma growth in min mice. in Whole Grain and Human Health: International Symposium: Abstracts. VTT Technical Research Centre of Finland, Espoo, VTT Symposium, no. 213, pp. 143-144, Whole Grain and Human Health: International Symposium
, Porvoo, Finland, 13/06/01.

Water soluble and insoluble fractions or rye bran have distinct effects on adenoma growth in min mice. / Oikarinen, S.; Päivärinta, E.; Sivula, A.; Karppinen, Sirpa; Poutanen, Kaisa; Mutanen, M.

Whole Grain and Human Health: International Symposium: Abstracts. Espoo : VTT Technical Research Centre of Finland, 2001. p. 143-144 (VTT Symposium; No. 213).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - Water soluble and insoluble fractions or rye bran have distinct effects on adenoma growth in min mice

AU - Oikarinen, S.

AU - Päivärinta, E.

AU - Sivula, A.

AU - Karppinen, Sirpa

AU - Poutanen, Kaisa

AU - Mutanen, M.

PY - 2001

Y1 - 2001

N2 - Rye is a promising candidate for anticarcinogenic components in human diet.Two recent studies using different animal models both suggest that rye bran may contain substances which prevent intestinal tumour formation in animals (Davies et al.Carcinogenesis 1999;20:927-931; Mutanen et al. Carcinogenesis 2000;21:1167-1173).In this study, we wanted to see if there exist differences between whole bran and water soluble (WS) and water insoluble (WIS) fractions of rye bran on intestinal adenoma formation in adenomatous polyposis coli (APC) knockout mice.These mice spontaneously develop multiple intestinal neoplasia (Min).Male heterozygous Min mice (n = 11-12/group) were fed one of the following high-fat AIN93G-based diets for five to six weeks: a casein based diet with 10% (w/w) rye bran, or 7.9% (w/w) WS or WIS fractions of rye bran.This way all three diets had the same amount of undigestible components (4.14%, w/w).In rye bran and in the WIS fraction dietary fiber and fructan contents were 34.3/7.1% and 51.1/1.2%, respectively. The WS fraction was prepared using xylanase treatment and it contained mainly fructan, pentosane-oligosacharides, and beta-glucan (21.2/26.7/4.4%).The fat (40% of energy) used in the diets was a mixture of butter, rapeseed oil, and sunflower seed oil.The intake of fatty acids corresponded to that in the Western type diet.The rye bran, WIS and WS diets produced similar number of adenomas in the small intestine (37±28; 38±34; 38±30, mean±SD) and in the colon+caecum (0.8±1.0; 1.0±1.1; 0.7±0.6).Opposite effect between WIS and WS diets were, however, seen on adenoma growth when comparing duodenum and the distal small intestine.In the duodenum the mean size of adenomas were 2.22±0.35 mm and 1.42±0.70 mm after the WIS and WS diets (p < 0.05, Mann-Whitney) while the corresponding values in the distal part of the small intestine were 0.99±0.21 mm and 1.20±0.17 mm (p < 0.05).The number of large (>1.5 mm) adenomas either in the duodenum or in the distal small intestine supported these results; in the duodenum WIS diet significantly increased the number of large adenomas (% of total) when compared with the whole rye bran (35±13% vs. 17±17%, p < 0.05), while it decreased the number of large adenomas in the distal small intestine (1.0±0.4% vs. 17±14%, p = 0.052).The results suggest that both WIS and WS fractions of rye bran probably contain substances which when separated from whole bran may, depending on the site of the intestine, have either favourable or unfavourable effects on the growth of adenomas in Min mice.

AB - Rye is a promising candidate for anticarcinogenic components in human diet.Two recent studies using different animal models both suggest that rye bran may contain substances which prevent intestinal tumour formation in animals (Davies et al.Carcinogenesis 1999;20:927-931; Mutanen et al. Carcinogenesis 2000;21:1167-1173).In this study, we wanted to see if there exist differences between whole bran and water soluble (WS) and water insoluble (WIS) fractions of rye bran on intestinal adenoma formation in adenomatous polyposis coli (APC) knockout mice.These mice spontaneously develop multiple intestinal neoplasia (Min).Male heterozygous Min mice (n = 11-12/group) were fed one of the following high-fat AIN93G-based diets for five to six weeks: a casein based diet with 10% (w/w) rye bran, or 7.9% (w/w) WS or WIS fractions of rye bran.This way all three diets had the same amount of undigestible components (4.14%, w/w).In rye bran and in the WIS fraction dietary fiber and fructan contents were 34.3/7.1% and 51.1/1.2%, respectively. The WS fraction was prepared using xylanase treatment and it contained mainly fructan, pentosane-oligosacharides, and beta-glucan (21.2/26.7/4.4%).The fat (40% of energy) used in the diets was a mixture of butter, rapeseed oil, and sunflower seed oil.The intake of fatty acids corresponded to that in the Western type diet.The rye bran, WIS and WS diets produced similar number of adenomas in the small intestine (37±28; 38±34; 38±30, mean±SD) and in the colon+caecum (0.8±1.0; 1.0±1.1; 0.7±0.6).Opposite effect between WIS and WS diets were, however, seen on adenoma growth when comparing duodenum and the distal small intestine.In the duodenum the mean size of adenomas were 2.22±0.35 mm and 1.42±0.70 mm after the WIS and WS diets (p < 0.05, Mann-Whitney) while the corresponding values in the distal part of the small intestine were 0.99±0.21 mm and 1.20±0.17 mm (p < 0.05).The number of large (>1.5 mm) adenomas either in the duodenum or in the distal small intestine supported these results; in the duodenum WIS diet significantly increased the number of large adenomas (% of total) when compared with the whole rye bran (35±13% vs. 17±17%, p < 0.05), while it decreased the number of large adenomas in the distal small intestine (1.0±0.4% vs. 17±14%, p = 0.052).The results suggest that both WIS and WS fractions of rye bran probably contain substances which when separated from whole bran may, depending on the site of the intestine, have either favourable or unfavourable effects on the growth of adenomas in Min mice.

M3 - Conference abstract in proceedings

SN - 951-38-5718-2

T3 - VTT Symposium

SP - 143

EP - 144

BT - Whole Grain and Human Health: International Symposium

PB - VTT Technical Research Centre of Finland

CY - Espoo

ER -

Oikarinen S, Päivärinta E, Sivula A, Karppinen S, Poutanen K, Mutanen M. Water soluble and insoluble fractions or rye bran have distinct effects on adenoma growth in min mice. In Whole Grain and Human Health: International Symposium: Abstracts. Espoo: VTT Technical Research Centre of Finland. 2001. p. 143-144. (VTT Symposium; No. 213).