Abstract
Rye is a promising candidate for anticarcinogenic components in human
diet.Two recent studies using different animal models both suggest that rye
bran may contain substances which prevent intestinal tumour formation in
animals (Davies et al.Carcinogenesis 1999;20:927-931; Mutanen et al.
Carcinogenesis 2000;21:1167-1173).In this study, we wanted to see if there
exist differences between whole bran and water soluble (WS) and water
insoluble (WIS) fractions of rye bran on intestinal adenoma formation in
adenomatous polyposis coli (APC) knockout mice.These mice spontaneously
develop multiple intestinal neoplasia (Min).Male heterozygous Min mice (n =
11-12/group) were fed one of the following high-fat AIN93G-based diets for
five to six weeks: a casein based diet with 10% (w/w) rye bran, or 7.9% (w/w)
WS or WIS fractions of rye bran.This way all three diets had the same amount
of undigestible components (4.14%, w/w).In rye bran and in the WIS fraction
dietary fiber and fructan contents were 34.3/7.1% and 51.1/1.2%, respectively.
The WS fraction was prepared using xylanase treatment and it contained mainly
fructan, pentosane-oligosacharides, and beta-glucan (21.2/26.7/4.4%).The fat
(40% of energy) used in the diets was a mixture of butter, rapeseed oil, and
sunflower seed oil.The intake of fatty acids corresponded to that in the
Western type diet.The rye bran, WIS and WS diets produced similar number of
adenomas in the small intestine (37±28; 38±34; 38±30, mean±SD) and in the
colon+caecum (0.8±1.0; 1.0±1.1; 0.7±0.6).Opposite effect between WIS and WS
diets were, however, seen on adenoma growth when comparing duodenum and the
distal small intestine.In the duodenum the mean size of adenomas were
2.22±0.35 mm and 1.42±0.70 mm after the WIS and WS diets (p < 0.05,
Mann-Whitney) while the corresponding values in the distal part of the small
intestine were 0.99±0.21 mm and 1.20±0.17 mm (p < 0.05).The number of large
(>1.5 mm) adenomas either in the duodenum or in the distal small intestine
supported these results; in the duodenum WIS diet significantly increased the
number of large adenomas (% of total) when compared with the whole rye bran
(35±13% vs. 17±17%, p < 0.05), while it decreased the number of large adenomas
in the distal small intestine (1.0±0.4% vs. 17±14%, p = 0.052).The results
suggest that both WIS and WS fractions of rye bran probably contain substances
which when separated from whole bran may, depending on the site of the
intestine, have either favourable or unfavourable effects on the growth of
adenomas in Min mice.
Original language | English |
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Title of host publication | Whole Grain and Human Health: International Symposium |
Subtitle of host publication | Abstracts |
Place of Publication | Espoo |
Publisher | VTT Technical Research Centre of Finland |
Pages | 143-144 |
ISBN (Electronic) | 951-38-5719-0 |
ISBN (Print) | 951-38-5718-2 |
Publication status | Published - 2001 |
MoE publication type | Not Eligible |
Event | Whole Grain and Human Health: International Symposium - Porvoo, Finland Duration: 13 Jun 2001 → 15 Jun 2001 |
Publication series
Series | VTT Symposium |
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Number | 213 |
ISSN | 0357-9387 |
Conference
Conference | Whole Grain and Human Health: International Symposium |
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Country/Territory | Finland |
City | Porvoo |
Period | 13/06/01 → 15/06/01 |