Wave-mixed and orbitally shaken single-use photobioreactors for diatom algae propagation

N. Lehmann (Corresponding Author), Heiko Rischer, D. Eibl, R. Eibl

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)

Abstract

Although vertical column, flat‐plate, tubular, bubble column and airlift photobioreactors are widely used for the cultivation of diatoms, it has been shown that bubble‐induced hydrodynamic stress created in these types of bioreactor can damage cells. Therefore, three single‐use surface‐aerated bag bioreactors, known for their outstanding results in cultivating shear‐sensitive mammalian cells, were for the first time investigated for their suitability in growing the model microalgae Phaeodactylum tricornutum. All of the systems, which were additionally aerated with CO2 and equipped with illumination systems providing different light qualities, guaranteed a 22‐ to 43‐fold increase in cell density within seven days, without any addition of cell protection agents or changes in cell morphology. Maximum cell density and dry biomass were achieved in the orbitally shaken 2D‐bag by using cool‐white fluorescent tubes.
Original languageEnglish
Pages (from-to)197-201
Number of pages5
JournalChemie-Ingenieur-Technik
Volume85
Issue number1-2
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fingerprint

Photobioreactors
Algae
Bioreactors
Bubble columns
Biomass
Hydrodynamics
Lighting
Cells

Keywords

  • diatom
  • growth characteristics
  • LED
  • orbitally shaken
  • phaeodactylum tricornutum
  • single-use bioreactor
  • wave-mixed

Cite this

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title = "Wave-mixed and orbitally shaken single-use photobioreactors for diatom algae propagation",
abstract = "Although vertical column, flat‐plate, tubular, bubble column and airlift photobioreactors are widely used for the cultivation of diatoms, it has been shown that bubble‐induced hydrodynamic stress created in these types of bioreactor can damage cells. Therefore, three single‐use surface‐aerated bag bioreactors, known for their outstanding results in cultivating shear‐sensitive mammalian cells, were for the first time investigated for their suitability in growing the model microalgae Phaeodactylum tricornutum. All of the systems, which were additionally aerated with CO2 and equipped with illumination systems providing different light qualities, guaranteed a 22‐ to 43‐fold increase in cell density within seven days, without any addition of cell protection agents or changes in cell morphology. Maximum cell density and dry biomass were achieved in the orbitally shaken 2D‐bag by using cool‐white fluorescent tubes.",
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Wave-mixed and orbitally shaken single-use photobioreactors for diatom algae propagation. / Lehmann, N. (Corresponding Author); Rischer, Heiko; Eibl, D.; Eibl, R.

In: Chemie-Ingenieur-Technik, Vol. 85, No. 1-2, 2013, p. 197-201.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Wave-mixed and orbitally shaken single-use photobioreactors for diatom algae propagation

AU - Lehmann, N.

AU - Rischer, Heiko

AU - Eibl, D.

AU - Eibl, R.

PY - 2013

Y1 - 2013

N2 - Although vertical column, flat‐plate, tubular, bubble column and airlift photobioreactors are widely used for the cultivation of diatoms, it has been shown that bubble‐induced hydrodynamic stress created in these types of bioreactor can damage cells. Therefore, three single‐use surface‐aerated bag bioreactors, known for their outstanding results in cultivating shear‐sensitive mammalian cells, were for the first time investigated for their suitability in growing the model microalgae Phaeodactylum tricornutum. All of the systems, which were additionally aerated with CO2 and equipped with illumination systems providing different light qualities, guaranteed a 22‐ to 43‐fold increase in cell density within seven days, without any addition of cell protection agents or changes in cell morphology. Maximum cell density and dry biomass were achieved in the orbitally shaken 2D‐bag by using cool‐white fluorescent tubes.

AB - Although vertical column, flat‐plate, tubular, bubble column and airlift photobioreactors are widely used for the cultivation of diatoms, it has been shown that bubble‐induced hydrodynamic stress created in these types of bioreactor can damage cells. Therefore, three single‐use surface‐aerated bag bioreactors, known for their outstanding results in cultivating shear‐sensitive mammalian cells, were for the first time investigated for their suitability in growing the model microalgae Phaeodactylum tricornutum. All of the systems, which were additionally aerated with CO2 and equipped with illumination systems providing different light qualities, guaranteed a 22‐ to 43‐fold increase in cell density within seven days, without any addition of cell protection agents or changes in cell morphology. Maximum cell density and dry biomass were achieved in the orbitally shaken 2D‐bag by using cool‐white fluorescent tubes.

KW - diatom

KW - growth characteristics

KW - LED

KW - orbitally shaken

KW - phaeodactylum tricornutum

KW - single-use bioreactor

KW - wave-mixed

U2 - 10.1002/cite.201200137

DO - 10.1002/cite.201200137

M3 - Article

VL - 85

SP - 197

EP - 201

JO - Chemie-Ingenieur-Technik

JF - Chemie-Ingenieur-Technik

SN - 0009-286X

IS - 1-2

ER -