Weakening effect of cell permeabilizers on gram-negative bacteria causing biodeterioration

Hanna Alakomi (Corresponding Author), Arja Paananen, Maija-Liisa Suihko, Ilkka Helander, Maria Saarela

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Gram-negative bacteria play an important role in the formation and stabilization of biofilm structures on stone surfaces. Therefore, the control of growth of gram-negative bacteria offers a way to diminish biodeterioration of stone materials. The effect of potential permeabilizers on the outer membrane (OM) properties of gram-negative bacteria was investigated and further characterized. In addition, efficacy of the agents in enhancing the activity of a biocide (benzalkonium chloride) was assessed. EDTA, polyethylenimine (PEI), and succimer (meso-2,3-dimercaptosuccinic) were shown to be efficient permeabilizers of the members of Pseudomonas and Stenotrophomonas genera, as indicated by an increase in the uptake of a hydrophobic probe (1-N-phenylnaphthylamine) and sensitization to hydrophobic antibiotics. Visualization of Pseudomonas cells treated with EDTA or PEI by atomic force microscopy revealed damage in the outer membrane structure. PEI especially increased the surface area and bulges of the cells. Topographic images of EDTA-treated cells were compatible with events assigned for the effect of EDTA on outer membranes, i.e., release of lipopolysaccharide and disintegration of OM structure. In addition, the effect of EDTA treatment was visualized in phase-contrast images as large areas with varying hydrophilicity on cell surfaces. In liquid culture tests, EDTA and PEI supplementation enhanced the activity of benzalkonium chloride toward the target strains. Use of permeabilizers in biocide formulations would enable the use of decreased concentrations of the active biocide ingredient, thereby providing environmentally friendlier products.
Original languageEnglish
Pages (from-to)4695-4703
Number of pages9
JournalApplied and Environmental Microbiology
Volume72
Issue number7
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed

Fingerprint

Gram-Negative Bacteria
EDTA
Gram-negative bacteria
Edetic Acid
biocides
Polyethyleneimine
biodegradation
benzalkonium chloride
bacterium
Disinfectants
Pseudomonas
Benzalkonium Compounds
membrane
Membranes
N-phenyl-1-naphthylamine
pesticide
Stenotrophomonas
cells
atomic force microscopy
chloride

Cite this

Alakomi, Hanna ; Paananen, Arja ; Suihko, Maija-Liisa ; Helander, Ilkka ; Saarela, Maria. / Weakening effect of cell permeabilizers on gram-negative bacteria causing biodeterioration. In: Applied and Environmental Microbiology. 2006 ; Vol. 72, No. 7. pp. 4695-4703.
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abstract = "Gram-negative bacteria play an important role in the formation and stabilization of biofilm structures on stone surfaces. Therefore, the control of growth of gram-negative bacteria offers a way to diminish biodeterioration of stone materials. The effect of potential permeabilizers on the outer membrane (OM) properties of gram-negative bacteria was investigated and further characterized. In addition, efficacy of the agents in enhancing the activity of a biocide (benzalkonium chloride) was assessed. EDTA, polyethylenimine (PEI), and succimer (meso-2,3-dimercaptosuccinic) were shown to be efficient permeabilizers of the members of Pseudomonas and Stenotrophomonas genera, as indicated by an increase in the uptake of a hydrophobic probe (1-N-phenylnaphthylamine) and sensitization to hydrophobic antibiotics. Visualization of Pseudomonas cells treated with EDTA or PEI by atomic force microscopy revealed damage in the outer membrane structure. PEI especially increased the surface area and bulges of the cells. Topographic images of EDTA-treated cells were compatible with events assigned for the effect of EDTA on outer membranes, i.e., release of lipopolysaccharide and disintegration of OM structure. In addition, the effect of EDTA treatment was visualized in phase-contrast images as large areas with varying hydrophilicity on cell surfaces. In liquid culture tests, EDTA and PEI supplementation enhanced the activity of benzalkonium chloride toward the target strains. Use of permeabilizers in biocide formulations would enable the use of decreased concentrations of the active biocide ingredient, thereby providing environmentally friendlier products.",
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Weakening effect of cell permeabilizers on gram-negative bacteria causing biodeterioration. / Alakomi, Hanna (Corresponding Author); Paananen, Arja; Suihko, Maija-Liisa; Helander, Ilkka; Saarela, Maria.

In: Applied and Environmental Microbiology, Vol. 72, No. 7, 2006, p. 4695-4703.

Research output: Contribution to journalArticleScientificpeer-review

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