Xylan as limiting factor in enzymatic hydrolysis of nanocellulose

P.A. Penttilä (Corresponding Author), Aniko Varnai, Jaakko Pere, Tekla Tammelin, L. Salmén, Matti Siika-aho, L. Viikari, R. Serimaa

Research output: Contribution to journalArticleScientificpeer-review

52 Citations (Scopus)

Abstract

The role of xylan as a limiting factor in the enzymatic hydrolysis of cellulose was studied by hydrolysing nanocellulose samples prepared by mechanical fibrillation of birch pulp with varying xylan content. Analyzing the nanocelluloses and their hydrolysis residues with dynamic FT-IR spectroscopy revealed that a certain fraction of xylan remained tightly attached to cellulose fibrils despite partial hydrolysis of xylan with xylanase prior to pulp fibrillation and that this fraction remained in the structure during the hydrolysis of nanocellulose with cellulase mixture as well. Thus, a loosely bound fraction of xylan was predicted to have been more likely removed by purified xylanase. The presence of loosely bound xylan seemed to limit the hydrolysis of crystalline cellulose, indicated by an increase in cellulose crystallinity and by preserved crystal width measured with wide-angle X-ray scattering. Removing loosely bound xylan led to a proportional hydrolysis of xylan and cellulose with the cellulase mixture.
Original languageEnglish
Pages (from-to)135-141
Number of pages7
JournalBioresource Technology
Volume129
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

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Xylans
Enzymatic hydrolysis
limiting factor
hydrolysis
cellulose
Cellulose
Hydrolysis
Cellulase
Pulp
crystallinity
X ray scattering
spectroscopy
scattering
crystal
Infrared spectroscopy
Crystalline materials
Crystals

Cite this

Penttilä, P.A. ; Varnai, Aniko ; Pere, Jaakko ; Tammelin, Tekla ; Salmén, L. ; Siika-aho, Matti ; Viikari, L. ; Serimaa, R. / Xylan as limiting factor in enzymatic hydrolysis of nanocellulose. In: Bioresource Technology. 2013 ; Vol. 129. pp. 135-141.
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abstract = "The role of xylan as a limiting factor in the enzymatic hydrolysis of cellulose was studied by hydrolysing nanocellulose samples prepared by mechanical fibrillation of birch pulp with varying xylan content. Analyzing the nanocelluloses and their hydrolysis residues with dynamic FT-IR spectroscopy revealed that a certain fraction of xylan remained tightly attached to cellulose fibrils despite partial hydrolysis of xylan with xylanase prior to pulp fibrillation and that this fraction remained in the structure during the hydrolysis of nanocellulose with cellulase mixture as well. Thus, a loosely bound fraction of xylan was predicted to have been more likely removed by purified xylanase. The presence of loosely bound xylan seemed to limit the hydrolysis of crystalline cellulose, indicated by an increase in cellulose crystallinity and by preserved crystal width measured with wide-angle X-ray scattering. Removing loosely bound xylan led to a proportional hydrolysis of xylan and cellulose with the cellulase mixture.",
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Xylan as limiting factor in enzymatic hydrolysis of nanocellulose. / Penttilä, P.A. (Corresponding Author); Varnai, Aniko; Pere, Jaakko; Tammelin, Tekla; Salmén, L.; Siika-aho, Matti; Viikari, L.; Serimaa, R.

In: Bioresource Technology, Vol. 129, 2013, p. 135-141.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Xylan as limiting factor in enzymatic hydrolysis of nanocellulose

AU - Penttilä, P.A.

AU - Varnai, Aniko

AU - Pere, Jaakko

AU - Tammelin, Tekla

AU - Salmén, L.

AU - Siika-aho, Matti

AU - Viikari, L.

AU - Serimaa, R.

N1 - CO:K University of Helsinki, Department of Physics CO:K University of Helsinki, Department of Food and Environmental Sciences CO:U Innventia AB CA2: TK404 CA2: TK511 SDA: AMA ISI: BIOTECHNOLOGY & APPLIED MICROBIOLOGY

PY - 2013

Y1 - 2013

N2 - The role of xylan as a limiting factor in the enzymatic hydrolysis of cellulose was studied by hydrolysing nanocellulose samples prepared by mechanical fibrillation of birch pulp with varying xylan content. Analyzing the nanocelluloses and their hydrolysis residues with dynamic FT-IR spectroscopy revealed that a certain fraction of xylan remained tightly attached to cellulose fibrils despite partial hydrolysis of xylan with xylanase prior to pulp fibrillation and that this fraction remained in the structure during the hydrolysis of nanocellulose with cellulase mixture as well. Thus, a loosely bound fraction of xylan was predicted to have been more likely removed by purified xylanase. The presence of loosely bound xylan seemed to limit the hydrolysis of crystalline cellulose, indicated by an increase in cellulose crystallinity and by preserved crystal width measured with wide-angle X-ray scattering. Removing loosely bound xylan led to a proportional hydrolysis of xylan and cellulose with the cellulase mixture.

AB - The role of xylan as a limiting factor in the enzymatic hydrolysis of cellulose was studied by hydrolysing nanocellulose samples prepared by mechanical fibrillation of birch pulp with varying xylan content. Analyzing the nanocelluloses and their hydrolysis residues with dynamic FT-IR spectroscopy revealed that a certain fraction of xylan remained tightly attached to cellulose fibrils despite partial hydrolysis of xylan with xylanase prior to pulp fibrillation and that this fraction remained in the structure during the hydrolysis of nanocellulose with cellulase mixture as well. Thus, a loosely bound fraction of xylan was predicted to have been more likely removed by purified xylanase. The presence of loosely bound xylan seemed to limit the hydrolysis of crystalline cellulose, indicated by an increase in cellulose crystallinity and by preserved crystal width measured with wide-angle X-ray scattering. Removing loosely bound xylan led to a proportional hydrolysis of xylan and cellulose with the cellulase mixture.

U2 - 10.1016/j.biortech.2012.11.017

DO - 10.1016/j.biortech.2012.11.017

M3 - Article

VL - 129

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JF - Bioresource Technology

SN - 0960-8524

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