Xylanases of thermophilic bacteria from Icelandic hot springs

Martti Perttula, Marjaana Rättö, Marta Konradsdottir, Jakob Kristjansson, Liisa Viikari

Research output: Contribution to journalArticleScientificpeer-review

21 Citations (Scopus)

Abstract

Thermophilic, aerobic bacteria isolated from Icelandic hot springs were screened for xylanase activity. Of 97 strains tested, 14 were found to be xylanase positive. Xylanase activities up to 12 nkat/ml were produced by these strains in shake flasks on xylan medium. The xylanases of the two strains producing the highest activities (ITI 36 and ITI 283) were similar with respect to temperature and pH optima (80°C and pH 8.0). Xylanase production of strain ITI 36 was found to be induced by xylan and xylose. Xylanase activity of 24 nkat/ml was obtained with this strain in a laboratory-scale-fermentor cultivation on xylose medium. β-Xylosidase activity was also detected in the culture filtrate. The thermal half-life of ITI 36 xylanase was 24 h at 70°C. The highest production of sugars from hydrolysis of beech xylan was obtained at 70°C, although xylan depolymerization was detected even up to 90°C.

Original languageEnglish
Pages (from-to)592 - 595
Number of pages4
JournalApplied Microbiology and Biotechnology
Volume38
Issue number5
DOIs
Publication statusPublished - 1993
MoE publication typeA1 Journal article-refereed

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Hot Springs
Xylans
Bacteria
Xylose
Xylosidases
Fagus
Aerobic Bacteria
Bioreactors
Half-Life
Hydrolysis
Hot Temperature
Temperature

Cite this

Perttula, M., Rättö, M., Konradsdottir, M., Kristjansson, J., & Viikari, L. (1993). Xylanases of thermophilic bacteria from Icelandic hot springs. Applied Microbiology and Biotechnology, 38(5), 592 - 595. https://doi.org/10.1007/BF00182794
Perttula, Martti ; Rättö, Marjaana ; Konradsdottir, Marta ; Kristjansson, Jakob ; Viikari, Liisa. / Xylanases of thermophilic bacteria from Icelandic hot springs. In: Applied Microbiology and Biotechnology. 1993 ; Vol. 38, No. 5. pp. 592 - 595.
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abstract = "Thermophilic, aerobic bacteria isolated from Icelandic hot springs were screened for xylanase activity. Of 97 strains tested, 14 were found to be xylanase positive. Xylanase activities up to 12 nkat/ml were produced by these strains in shake flasks on xylan medium. The xylanases of the two strains producing the highest activities (ITI 36 and ITI 283) were similar with respect to temperature and pH optima (80°C and pH 8.0). Xylanase production of strain ITI 36 was found to be induced by xylan and xylose. Xylanase activity of 24 nkat/ml was obtained with this strain in a laboratory-scale-fermentor cultivation on xylose medium. β-Xylosidase activity was also detected in the culture filtrate. The thermal half-life of ITI 36 xylanase was 24 h at 70°C. The highest production of sugars from hydrolysis of beech xylan was obtained at 70°C, although xylan depolymerization was detected even up to 90°C.",
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Perttula, M, Rättö, M, Konradsdottir, M, Kristjansson, J & Viikari, L 1993, 'Xylanases of thermophilic bacteria from Icelandic hot springs', Applied Microbiology and Biotechnology, vol. 38, no. 5, pp. 592 - 595. https://doi.org/10.1007/BF00182794

Xylanases of thermophilic bacteria from Icelandic hot springs. / Perttula, Martti; Rättö, Marjaana; Konradsdottir, Marta; Kristjansson, Jakob; Viikari, Liisa.

In: Applied Microbiology and Biotechnology, Vol. 38, No. 5, 1993, p. 592 - 595.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Xylanases of thermophilic bacteria from Icelandic hot springs

AU - Perttula, Martti

AU - Rättö, Marjaana

AU - Konradsdottir, Marta

AU - Kristjansson, Jakob

AU - Viikari, Liisa

N1 - Project code: BIO0110

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N2 - Thermophilic, aerobic bacteria isolated from Icelandic hot springs were screened for xylanase activity. Of 97 strains tested, 14 were found to be xylanase positive. Xylanase activities up to 12 nkat/ml were produced by these strains in shake flasks on xylan medium. The xylanases of the two strains producing the highest activities (ITI 36 and ITI 283) were similar with respect to temperature and pH optima (80°C and pH 8.0). Xylanase production of strain ITI 36 was found to be induced by xylan and xylose. Xylanase activity of 24 nkat/ml was obtained with this strain in a laboratory-scale-fermentor cultivation on xylose medium. β-Xylosidase activity was also detected in the culture filtrate. The thermal half-life of ITI 36 xylanase was 24 h at 70°C. The highest production of sugars from hydrolysis of beech xylan was obtained at 70°C, although xylan depolymerization was detected even up to 90°C.

AB - Thermophilic, aerobic bacteria isolated from Icelandic hot springs were screened for xylanase activity. Of 97 strains tested, 14 were found to be xylanase positive. Xylanase activities up to 12 nkat/ml were produced by these strains in shake flasks on xylan medium. The xylanases of the two strains producing the highest activities (ITI 36 and ITI 283) were similar with respect to temperature and pH optima (80°C and pH 8.0). Xylanase production of strain ITI 36 was found to be induced by xylan and xylose. Xylanase activity of 24 nkat/ml was obtained with this strain in a laboratory-scale-fermentor cultivation on xylose medium. β-Xylosidase activity was also detected in the culture filtrate. The thermal half-life of ITI 36 xylanase was 24 h at 70°C. The highest production of sugars from hydrolysis of beech xylan was obtained at 70°C, although xylan depolymerization was detected even up to 90°C.

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Perttula M, Rättö M, Konradsdottir M, Kristjansson J, Viikari L. Xylanases of thermophilic bacteria from Icelandic hot springs. Applied Microbiology and Biotechnology. 1993;38(5):592 - 595. https://doi.org/10.1007/BF00182794