Xylitol production by recombinant saccharomyces cerevisiae

Johan Hallborn, Mats Walfridsson, Ulla Airaksinen, Heikki Ojamo, Bärbel Hahn-Hägerdal, Merja Penttilä, S. Keränen

Research output: Contribution to journalArticleScientificpeer-review

148 Citations (Scopus)

Abstract

We obtained efficient conversion of xylose to xylitol by transforming Saccharomyces cerevisiae with the gene encoding the xylose reductase (XR) of Pichia stipitis CBS 6054. Comparison of the chromosomal and cDNA copies of the XYL1 gene showed that the genomic XYL1 contains no introns, and an XR monomer of 318 amino acids (35, 985 D) is encoded by an open reading frame of 954 bp. The amino acid sequence of the P. stipitis XR is similar to several aldose reductases, suggesting that P. stipitis XR is part of the aldoketo reductase superfamily. S. cerevisiae transformed with the XYL1 gene gave over 95% conversion of xylose into xylitol, a yield not obtainable with natural xylose utilizing yeasts.

Original languageEnglish
Pages (from-to)1090-1095
Number of pages6
JournalBio/Technology
Volume9
Issue number11
DOIs
Publication statusPublished - 1 Jan 1991
MoE publication typeA1 Journal article-refereed

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Xylitol
Aldehyde Reductase
Xylose
Yeast
Saccharomyces cerevisiae
Genes
Amino Acids
Amino acids
Gene encoding
Pichia
Introns
Open Reading Frames
Amino Acid Sequence
Oxidoreductases
Complementary DNA
Yeasts
Monomers

Cite this

Hallborn, J., Walfridsson, M., Airaksinen, U., Ojamo, H., Hahn-Hägerdal, B., Penttilä, M., & Keränen, S. (1991). Xylitol production by recombinant saccharomyces cerevisiae. Bio/Technology, 9(11), 1090-1095. https://doi.org/10.1038/nbt1191-1090
Hallborn, Johan ; Walfridsson, Mats ; Airaksinen, Ulla ; Ojamo, Heikki ; Hahn-Hägerdal, Bärbel ; Penttilä, Merja ; Keränen, S. / Xylitol production by recombinant saccharomyces cerevisiae. In: Bio/Technology. 1991 ; Vol. 9, No. 11. pp. 1090-1095.
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Hallborn, J, Walfridsson, M, Airaksinen, U, Ojamo, H, Hahn-Hägerdal, B, Penttilä, M & Keränen, S 1991, 'Xylitol production by recombinant saccharomyces cerevisiae', Bio/Technology, vol. 9, no. 11, pp. 1090-1095. https://doi.org/10.1038/nbt1191-1090

Xylitol production by recombinant saccharomyces cerevisiae. / Hallborn, Johan; Walfridsson, Mats; Airaksinen, Ulla; Ojamo, Heikki; Hahn-Hägerdal, Bärbel; Penttilä, Merja; Keränen, S.

In: Bio/Technology, Vol. 9, No. 11, 01.01.1991, p. 1090-1095.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Hallborn, Johan

AU - Walfridsson, Mats

AU - Airaksinen, Ulla

AU - Ojamo, Heikki

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AU - Penttilä, Merja

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Hallborn J, Walfridsson M, Airaksinen U, Ojamo H, Hahn-Hägerdal B, Penttilä M et al. Xylitol production by recombinant saccharomyces cerevisiae. Bio/Technology. 1991 Jan 1;9(11):1090-1095. https://doi.org/10.1038/nbt1191-1090