TY - JOUR
T1 - Xylitol production by recombinant saccharomyces cerevisiae
AU - Hallborn, Johan
AU - Walfridsson, Mats
AU - Airaksinen, Ulla
AU - Ojamo, Heikki
AU - Hahn-Hägerdal, Bärbel
AU - Penttilä, Merja
AU - Keränen, Sirkka
PY - 1991/1/1
Y1 - 1991/1/1
N2 - We obtained efficient conversion of xylose to xylitol by transforming Saccharomyces cerevisiae with the gene encoding the xylose reductase (XR) of Pichia stipitis CBS 6054. Comparison of the chromosomal and cDNA copies of the XYL1 gene showed that the genomic XYL1 contains no introns, and an XR monomer of 318 amino acids (35, 985 D) is encoded by an open reading frame of 954 bp. The amino acid sequence of the P. stipitis XR is similar to several aldose reductases, suggesting that P. stipitis XR is part of the aldoketo reductase superfamily. S. cerevisiae transformed with the XYL1 gene gave over 95% conversion of xylose into xylitol, a yield not obtainable with natural xylose utilizing yeasts.
AB - We obtained efficient conversion of xylose to xylitol by transforming Saccharomyces cerevisiae with the gene encoding the xylose reductase (XR) of Pichia stipitis CBS 6054. Comparison of the chromosomal and cDNA copies of the XYL1 gene showed that the genomic XYL1 contains no introns, and an XR monomer of 318 amino acids (35, 985 D) is encoded by an open reading frame of 954 bp. The amino acid sequence of the P. stipitis XR is similar to several aldose reductases, suggesting that P. stipitis XR is part of the aldoketo reductase superfamily. S. cerevisiae transformed with the XYL1 gene gave over 95% conversion of xylose into xylitol, a yield not obtainable with natural xylose utilizing yeasts.
UR - http://www.scopus.com/inward/record.url?scp=0026317988&partnerID=8YFLogxK
U2 - 10.1038/nbt1191-1090
DO - 10.1038/nbt1191-1090
M3 - Article
C2 - 1367625
SN - 1087-0156
VL - 9
SP - 1090
EP - 1095
JO - Nature Biotechnology
JF - Nature Biotechnology
IS - 11
ER -