Xylitol production by recombinant saccharomyces cerevisiae

Johan Hallborn; Mats Walfridsson; Ulla Airaksinen; Heikki Ojamo; Bärbel Hahn-Hägerdal; Merja Penttilä; Sirkka Keränen

doi:10.1038/nbt1191-1090

Xylitol production by recombinant saccharomyces cerevisiae

Johan Hallborn^*, Mats Walfridsson, Ulla Airaksinen, Heikki Ojamo, Bärbel Hahn-Hägerdal, Merja Penttilä, Sirkka Keränen

^*Corresponding author for this work

Lund University

Research output: Contribution to journal › Article › Scientific › peer-review

174 Citations (Scopus)

Abstract

We obtained efficient conversion of xylose to xylitol by transforming Saccharomyces cerevisiae with the gene encoding the xylose reductase (XR) of Pichia stipitis CBS 6054. Comparison of the chromosomal and cDNA copies of the XYL1 gene showed that the genomic XYL1 contains no introns, and an XR monomer of 318 amino acids (35, 985 D) is encoded by an open reading frame of 954 bp. The amino acid sequence of the P. stipitis XR is similar to several aldose reductases, suggesting that P. stipitis XR is part of the aldoketo reductase superfamily. S. cerevisiae transformed with the XYL1 gene gave over 95% conversion of xylose into xylitol, a yield not obtainable with natural xylose utilizing yeasts.

Original language	English
Pages (from-to)	1090-1095
Journal	Bio/Technology
Volume	9
Issue number	11
DOIs	https://doi.org/10.1038/nbt1191-1090
Publication status	Published - 1 Jan 1991
MoE publication type	A1 Journal article-refereed

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abstract = "We obtained efficient conversion of xylose to xylitol by transforming Saccharomyces cerevisiae with the gene encoding the xylose reductase (XR) of Pichia stipitis CBS 6054. Comparison of the chromosomal and cDNA copies of the XYL1 gene showed that the genomic XYL1 contains no introns, and an XR monomer of 318 amino acids (35, 985 D) is encoded by an open reading frame of 954 bp. The amino acid sequence of the P. stipitis XR is similar to several aldose reductases, suggesting that P. stipitis XR is part of the aldoketo reductase superfamily. S. cerevisiae transformed with the XYL1 gene gave over 95% conversion of xylose into xylitol, a yield not obtainable with natural xylose utilizing yeasts.",

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AU - Hallborn, Johan

AU - Walfridsson, Mats

AU - Airaksinen, Ulla

AU - Ojamo, Heikki

AU - Hahn-Hägerdal, Bärbel

AU - Penttilä, Merja

AU - Keränen, Sirkka

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N2 - We obtained efficient conversion of xylose to xylitol by transforming Saccharomyces cerevisiae with the gene encoding the xylose reductase (XR) of Pichia stipitis CBS 6054. Comparison of the chromosomal and cDNA copies of the XYL1 gene showed that the genomic XYL1 contains no introns, and an XR monomer of 318 amino acids (35, 985 D) is encoded by an open reading frame of 954 bp. The amino acid sequence of the P. stipitis XR is similar to several aldose reductases, suggesting that P. stipitis XR is part of the aldoketo reductase superfamily. S. cerevisiae transformed with the XYL1 gene gave over 95% conversion of xylose into xylitol, a yield not obtainable with natural xylose utilizing yeasts.

AB - We obtained efficient conversion of xylose to xylitol by transforming Saccharomyces cerevisiae with the gene encoding the xylose reductase (XR) of Pichia stipitis CBS 6054. Comparison of the chromosomal and cDNA copies of the XYL1 gene showed that the genomic XYL1 contains no introns, and an XR monomer of 318 amino acids (35, 985 D) is encoded by an open reading frame of 954 bp. The amino acid sequence of the P. stipitis XR is similar to several aldose reductases, suggesting that P. stipitis XR is part of the aldoketo reductase superfamily. S. cerevisiae transformed with the XYL1 gene gave over 95% conversion of xylose into xylitol, a yield not obtainable with natural xylose utilizing yeasts.

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Xylitol production by recombinant saccharomyces cerevisiae

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