Yeast as a tool to express sugar acid transporters with biotechnological interest

David Ribas, Joana Sá-Pessoa, Isabel Soares-Silva, Sandra Paiva, Yvonne Nygård, Laura Ruohonen, Merja Penttilä, Margarida Casal

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)

Abstract

Sugar acids can be used as platform chemicals to generate primary building blocks of industrially relevant products. Microbial production of these organic compounds at high yields requires the engineering of the enzymatic machinery and the presence of plasma membrane transporters able to export them outside the cells. In this study, several yeast carboxylic acid transporters belonging to the Jen family were screened for the transport of biotechnologically relevant sugar acids, namely gluconic, saccharic, mucic, xylaric and xylonic acid, and functionally characterised in Saccharomyces cerevisiae. We show that Jen permeases are capable of transporting most of these sugar acids, although with different specificities. Saccharate is a substrate of the transporters ScJen1-S271Q and KlJen2, gluconate of CaJen2 and KlJen2, and xylarate and mucate of CaJen2. A molecular docking approach of these transporters identified the residues that play a major role in the substrate binding of these sugar acids, namely R188 (ScJen1), R122 (CaJen2) and R127 (KlJen2), all equivalent residues (TMS II). The identification of Jen members as sugar acid transporters can contribute to engineering efficient microbial cell factories with increased sugar acid production, as the ScJen1 is able to promote substrate efflux.
Original languageEnglish
Article numberfox005
JournalFEMS Yeast Research
Volume17
Issue number2
DOIs
Publication statusPublished - 1 Mar 2017
MoE publication typeA1 Journal article-refereed

Fingerprint

Sugar Acids
Yeasts
Membrane Transport Proteins
Carboxylic Acids
Saccharomyces cerevisiae
Cell Membrane
Acids

Keywords

  • carboxylate transporters
  • Jen family
  • microbial production of acids
  • sugar acids
  • yeast

Cite this

Ribas, D., Sá-Pessoa, J., Soares-Silva, I., Paiva, S., Nygård, Y., Ruohonen, L., ... Casal, M. (2017). Yeast as a tool to express sugar acid transporters with biotechnological interest. FEMS Yeast Research, 17(2), [fox005]. https://doi.org/10.1093/femsyr/fox005
Ribas, David ; Sá-Pessoa, Joana ; Soares-Silva, Isabel ; Paiva, Sandra ; Nygård, Yvonne ; Ruohonen, Laura ; Penttilä, Merja ; Casal, Margarida. / Yeast as a tool to express sugar acid transporters with biotechnological interest. In: FEMS Yeast Research. 2017 ; Vol. 17, No. 2.
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Ribas, D, Sá-Pessoa, J, Soares-Silva, I, Paiva, S, Nygård, Y, Ruohonen, L, Penttilä, M & Casal, M 2017, 'Yeast as a tool to express sugar acid transporters with biotechnological interest', FEMS Yeast Research, vol. 17, no. 2, fox005. https://doi.org/10.1093/femsyr/fox005

Yeast as a tool to express sugar acid transporters with biotechnological interest. / Ribas, David; Sá-Pessoa, Joana; Soares-Silva, Isabel; Paiva, Sandra; Nygård, Yvonne; Ruohonen, Laura; Penttilä, Merja; Casal, Margarida.

In: FEMS Yeast Research, Vol. 17, No. 2, fox005, 01.03.2017.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Sá-Pessoa, Joana

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AU - Ruohonen, Laura

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AU - Casal, Margarida

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AB - Sugar acids can be used as platform chemicals to generate primary building blocks of industrially relevant products. Microbial production of these organic compounds at high yields requires the engineering of the enzymatic machinery and the presence of plasma membrane transporters able to export them outside the cells. In this study, several yeast carboxylic acid transporters belonging to the Jen family were screened for the transport of biotechnologically relevant sugar acids, namely gluconic, saccharic, mucic, xylaric and xylonic acid, and functionally characterised in Saccharomyces cerevisiae. We show that Jen permeases are capable of transporting most of these sugar acids, although with different specificities. Saccharate is a substrate of the transporters ScJen1-S271Q and KlJen2, gluconate of CaJen2 and KlJen2, and xylarate and mucate of CaJen2. A molecular docking approach of these transporters identified the residues that play a major role in the substrate binding of these sugar acids, namely R188 (ScJen1), R122 (CaJen2) and R127 (KlJen2), all equivalent residues (TMS II). The identification of Jen members as sugar acid transporters can contribute to engineering efficient microbial cell factories with increased sugar acid production, as the ScJen1 is able to promote substrate efflux.

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Ribas D, Sá-Pessoa J, Soares-Silva I, Paiva S, Nygård Y, Ruohonen L et al. Yeast as a tool to express sugar acid transporters with biotechnological interest. FEMS Yeast Research. 2017 Mar 1;17(2). fox005. https://doi.org/10.1093/femsyr/fox005