Abstract
A diffraction phase microscopy approach with a combined use of transmission and reflection imaging modes has been developed and applied for non-invasive quantitative assessment of the refractive index of red blood cells (RBCs). We present the theoretical background of signal formation for both imaging modes, accompanied by the results of experimental studies. We demonstrate that simultaneous use of the two modes has great potential for accurate assessment of the refractive index of biological cells, and we perform a reconstruction of spatial distribution of the refractive index of RBC in 3D.
| Original language | English |
|---|---|
| Article number | 105601 |
| Journal | Laser Physics Letters |
| Volume | 14 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 2017 |
| MoE publication type | A1 Journal article-refereed |
Funding
The authors acknowledge the support provided by CIMO Foundation (CIMO Fellowship, No. TM-15-9625), as well as partial support provided by The Ministry of Science and Education of the Russian Federation, No. 3.1340.2014/K, grant project ‘Development of optical methods and instrumentation for measurements and control of structure and dynamics of biological media’, government contract No. 2014/203 and by Government of Russian Federation, Grant 074-U01. Professor Meglinski also acknowledges partial support provided by Russian Science Foundation project No.15-14-10008.
Keywords
- cell imaging
- diffraction phase microscopy
- micro-interferometry
- quantitative phase imaging
- red blood cells
- refractive index